Schema for R-Loop DRIP-Seq (S2) - R-Loop DRIP-Seq in S2 Cells
  Database: dm6    Primary Table: CSE99014_dH1_RNH1_treated_idr    Row Count: 51   Data last updated: 2021-09-01
fieldexampleSQL type info
bin 680smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 12493435int(10) unsigned range
chromEnd 12493800int(10) unsigned range
name .varchar(255) values
score 697int(10) unsigned range
strand .char(1) values
signalValue 2.64329float range
pValue 12.9814float range
qValue 8.99452float range
peak 238int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
680chr2L1249343512493800.697.2.6432912.98148.99452238
600chr2R20165602017053.994.1.252521.033250.04721374
600chr2R20165602017053.994.2.7536615.348311.2664143
615chr2R40140904015289.1000.8.68533142.466137.641429
615chr2R40140904015289.1000.9.44043156.67151.258665
615chr2R40140904015289.1000.9.53919146.524141.603975
687chr2R1348564913486405.1000.2.8219215.081511.0038642
687chr2R1348564913486405.1000.2.9887318.692614.5781158
687chr2R1348564913486405.1000.3.3297622.1618.0167400
722chr2R1802527818025831.585.1.32791.281760.13231119

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

R-Loop DRIP-Seq (S2) (rloopDRIPSeq) Track Description
 

Description

These tracks show the putative locations of R-loops based on DRIP-Seq data from Drosophila melanogaster S2 Cells. The samples that were treated with RNase H served as additional controls for identifying R-loops. The regions that are enriched in the DRIP-Seq reads were identified by MACS2 and Peakzilla. The log likelihood ratio evidence tracks were produced by MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The DRIP-Seq datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession number GSE99014.

References

Bayona-Feliu A, Casas-Lamesa A, Reina O, Bernués J, Azorin F. Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin. Nat Commun. 2017 Aug 18;8(1):283.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.