Schema for R-Loop DRIP-Seq (S2) - R-Loop DRIP-Seq in S2 Cells
  Database: dm6    Primary Table: CSE99014_dH1_RNH1_treated_macs    Row Count: 142   Data last updated: 2021-09-01
fieldexampleSQL type info
bin 589smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 626860int(10) unsigned range
chromEnd 627148int(10) unsigned range
name CSE99014_dH1_RNH1_treated_m...varchar(255) values
score 47int(10) unsigned range
strand .char(1) values
signalValue 2.36114float range
pValue 8.41826float range
qValue 4.79052float range
peak 217int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
589chr2L626860627148CSE99014_dH1_RNH1_treated_macs_peak_1b47.2.361148.418264.79052217
589chr2L626860627148CSE99014_dH1_RNH1_treated_macs_peak_1a32.2.17866.810393.2854664
680chr2L1249360412493749CSE99014_dH1_RNH1_treated_macs_peak_289.2.6432912.98148.9945269
763chr2L2337092623371135CSE99014_dH1_RNH1_treated_macs_peak_331.1.523116.695063.1826589
591chr2R870255870560CSE99014_dH1_RNH1_treated_macs_peak_463.1.69839.98456.30015171
600chr2R20166132016778CSE99014_dH1_RNH1_treated_macs_peak_5112.2.7536615.348311.266490
615chr2R40141104015271CSE99014_dH1_RNH1_treated_macs_peak_6b1512.9.44043156.67151.258645
615chr2R40141104015271CSE99014_dH1_RNH1_treated_macs_peak_6a1376.8.68533142.466137.641409
615chr2R40141104015271CSE99014_dH1_RNH1_treated_macs_peak_6c1416.9.53919146.524141.603955
661chr2R1005775710057915CSE99014_dH1_RNH1_treated_macs_peak_776.2.3751511.42837.6174473

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

R-Loop DRIP-Seq (S2) (rloopDRIPSeq) Track Description
 

Description

These tracks show the putative locations of R-loops based on DRIP-Seq data from Drosophila melanogaster S2 Cells. The samples that were treated with RNase H served as additional controls for identifying R-loops. The regions that are enriched in the DRIP-Seq reads were identified by MACS2 and Peakzilla. The log likelihood ratio evidence tracks were produced by MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The DRIP-Seq datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession number GSE99014.

References

Bayona-Feliu A, Casas-Lamesa A, Reina O, Bernués J, Azorin F. Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin. Nat Commun. 2017 Aug 18;8(1):283.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.