Schema for R-Loop DRIP-Seq (S2) - R-Loop DRIP-Seq in S2 Cells
  Database: dm6    Primary Table: CSE99014_dH1_RNH1_untreated_idr    Row Count: 1,184   Data last updated: 2021-09-01
fieldexampleSQL type info
bin 586smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 192081int(10) unsigned range
chromEnd 193057int(10) unsigned range
name .varchar(255) values
score 564int(10) unsigned range
strand .char(1) values
signalValue 1.49569float range
pValue 1.51739float range
qValue 0.6627float range
peak 122int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
586chr2L192081193057.564.1.495691.517390.6627122
586chr2L192081193057.564.2.031443.620662.40987859
586chr2L192081193057.564.2.805047.912226.1916318
586chr2L192081193057.564.2.879588.398236.62904420
586chr2L192081193057.564.4.8252423.985221.1704561
588chr2L521042521444.1000.6.7910667.694363.045277
588chr2L521042521444.1000.1.660692.800771.7105678
589chr2L626381628143.1000.3.5153916.646814.2369126
589chr2L626381628143.1000.4.1090519.414916.84321250
589chr2L626381628143.1000.4.6103528.772625.72921522

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

R-Loop DRIP-Seq (S2) (rloopDRIPSeq) Track Description
 

Description

These tracks show the putative locations of R-loops based on DRIP-Seq data from Drosophila melanogaster S2 Cells. The samples that were treated with RNase H served as additional controls for identifying R-loops. The regions that are enriched in the DRIP-Seq reads were identified by MACS2 and Peakzilla. The log likelihood ratio evidence tracks were produced by MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The DRIP-Seq datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession number GSE99014.

References

Bayona-Feliu A, Casas-Lamesa A, Reina O, Bernués J, Azorin F. Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin. Nat Commun. 2017 Aug 18;8(1):283.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.