Schema for R-Loop DRIP-Seq (S2) - R-Loop DRIP-Seq in S2 Cells
  Database: dm6    Primary Table: CSE99014_dH1_RNH1_untreated_peakzilla    Row Count: 26,508   Data last updated: 2021-09-01
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 5436int(10) unsigned range
chromEnd 5730int(10) unsigned range
name peakzilla_18508varchar(255) values
score 2int(10) unsigned range
strand .char(1) values
signalValue 2.88float range
pValue -1float range
qValue 1.62342float range
peak 147int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L54365730peakzilla_185082.2.88-11.62342147
585chr2L57466040peakzilla_21716.4.62-12.38722147
585chr2L985310147peakzilla_164183.5.3-11.72354147
585chr2L1684717141peakzilla_148813.4.28-11.77469147
585chr2L1967719971peakzilla_102693.6.39-11.93181147
585chr2L4727047564peakzilla_177962.2.19-11.65758147
585chr2L102483102777peakzilla_175772.3.17-11.67162147
585chr2L116449116743peakzilla_194562.3.62-11.57512147
585chr2L123355123649peakzilla_177422.3.02-11.66555147
586chr2L132283132577peakzilla_156253.2.97-11.74232147

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

R-Loop DRIP-Seq (S2) (rloopDRIPSeq) Track Description
 

Description

These tracks show the putative locations of R-loops based on DRIP-Seq data from Drosophila melanogaster S2 Cells. The samples that were treated with RNase H served as additional controls for identifying R-loops. The regions that are enriched in the DRIP-Seq reads were identified by MACS2 and Peakzilla. The log likelihood ratio evidence tracks were produced by MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The DRIP-Seq datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession number GSE99014.

References

Bayona-Feliu A, Casas-Lamesa A, Reina O, Bernués J, Azorin F. Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin. Nat Commun. 2017 Aug 18;8(1):283.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.