Schema for R-Loop DRIP-Seq (S2) - R-Loop DRIP-Seq in S2 Cells
  Database: dm6    Primary Table: CSE99014_wt_RNH1_treated_idr    Row Count: 543   Data last updated: 2021-09-01
fieldexampleSQL type info
bin 589smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 626435int(10) unsigned range
chromEnd 628076int(10) unsigned range
name .varchar(255) values
score 1000int(10) unsigned range
strand .char(1) values
signalValue 7.80301float range
pValue 55.2878float range
qValue 51.0166float range
peak 652int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
589chr2L626435628076.1000.7.8030155.287851.0166652
589chr2L626435628076.1000.6.1277336.675832.9254382
589chr2L626435628076.1000.4.2870718.52314.91471403
589chr2L626435628076.1000.3.5459412.73529.208881153
593chr2L10773651077620.575.2.49366.453943.35599122
593chr2L11205081120756.549.2.612087.255884.03062153
595chr2L14117891412001.695.2.7836212.74119.21463123
601chr2L21756192175839.627.2.695747.133653.9222189
601chr2L22034142203600.604.2.302774.548121.9786889
603chr2L23784212378877.1000.3.099898.90345.51466136

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

R-Loop DRIP-Seq (S2) (rloopDRIPSeq) Track Description
 

Description

These tracks show the putative locations of R-loops based on DRIP-Seq data from Drosophila melanogaster S2 Cells. The samples that were treated with RNase H served as additional controls for identifying R-loops. The regions that are enriched in the DRIP-Seq reads were identified by MACS2 and Peakzilla. The log likelihood ratio evidence tracks were produced by MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The DRIP-Seq datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession number GSE99014.

References

Bayona-Feliu A, Casas-Lamesa A, Reina O, Bernués J, Azorin F. Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin. Nat Commun. 2017 Aug 18;8(1):283.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.