Schema for R-Loop DRIP-Seq (S2) - R-Loop DRIP-Seq in S2 Cells
  Database: dm6    Primary Table: CSE99014_wt_RNH1_treated_macs    Row Count: 626   Data last updated: 2021-09-01
fieldexampleSQL type info
bin 588smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 477208int(10) unsigned range
chromEnd 477337int(10) unsigned range
name CSE99014_wt_RNH1_treated_ma...varchar(255) values
score 36int(10) unsigned range
strand .char(1) values
signalValue 2.11758float range
pValue 6.8365float range
qValue 3.66663float range
peak 117int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
588chr2L477208477337CSE99014_wt_RNH1_treated_macs_peak_136.2.117586.83653.66663117
588chr2L502820503052CSE99014_wt_RNH1_treated_macs_peak_261.2.208199.613576.18277192
589chr2L561081561261CSE99014_wt_RNH1_treated_macs_peak_340.2.606717.232524.0094270
589chr2L626479628019CSE99014_wt_RNH1_treated_macs_peak_4a329.6.1277336.675832.9254338
589chr2L626479628019CSE99014_wt_RNH1_treated_macs_peak_4b510.7.8030155.287851.0166608
589chr2L626479628019CSE99014_wt_RNH1_treated_macs_peak_4c92.3.5459412.73529.208881109
589chr2L626479628019CSE99014_wt_RNH1_treated_macs_peak_4d149.4.2870718.52314.91471359
593chr2L11589481159125CSE99014_wt_RNH1_treated_macs_peak_560.2.480819.456266.0323866
595chr2L13945901394715CSE99014_wt_RNH1_treated_macs_peak_642.2.27357.503764.240975
595chr2L14118481411977CSE99014_wt_RNH1_treated_macs_peak_792.2.7836212.74119.2146364

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

R-Loop DRIP-Seq (S2) (rloopDRIPSeq) Track Description
 

Description

These tracks show the putative locations of R-loops based on DRIP-Seq data from Drosophila melanogaster S2 Cells. The samples that were treated with RNase H served as additional controls for identifying R-loops. The regions that are enriched in the DRIP-Seq reads were identified by MACS2 and Peakzilla. The log likelihood ratio evidence tracks were produced by MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The DRIP-Seq datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession number GSE99014.

References

Bayona-Feliu A, Casas-Lamesa A, Reina O, Bernués J, Azorin F. Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin. Nat Commun. 2017 Aug 18;8(1):283.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.