Schema for R-Loop DRIP-Seq (S2) - R-Loop DRIP-Seq in S2 Cells
  Database: dm6    Primary Table: CSE99014_wt_RNH1_treated_peakzilla    Row Count: 9,306   Data last updated: 2021-09-01
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 123395int(10) unsigned range
chromEnd 123649int(10) unsigned range
name peakzilla_13403varchar(255) values
score 1int(10) unsigned range
strand .char(1) values
signalValue 2.05float range
pValue -1float range
qValue 1.11014float range
peak 127int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L123395123649peakzilla_134031.2.05-11.11014127
586chr2L143879144133peakzilla_42272.2.5-11.4437127
586chr2L166389166643peakzilla_59192.3.05-11.36957127
586chr2L189950190204peakzilla_24472.3.03-11.52143127
586chr2L190238190492peakzilla_122341.2.3-11.14935127
586chr2L221921222175peakzilla_144641.2.1-11.07727127
586chr2L248249248503peakzilla_57592.2.94-11.36957127
587chr2L273342273596peakzilla_76491.2.64-11.29414127
587chr2L282258282512peakzilla_38242.3.22-11.4698127
587chr2L290412290666peakzilla_65572.2.65-11.34104127

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

R-Loop DRIP-Seq (S2) (rloopDRIPSeq) Track Description
 

Description

These tracks show the putative locations of R-loops based on DRIP-Seq data from Drosophila melanogaster S2 Cells. The samples that were treated with RNase H served as additional controls for identifying R-loops. The regions that are enriched in the DRIP-Seq reads were identified by MACS2 and Peakzilla. The log likelihood ratio evidence tracks were produced by MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The DRIP-Seq datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession number GSE99014.

References

Bayona-Feliu A, Casas-Lamesa A, Reina O, Bernués J, Azorin F. Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin. Nat Commun. 2017 Aug 18;8(1):283.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.