Schema for R-Loop DRIP-Seq (S2) - R-Loop DRIP-Seq in S2 Cells
  Database: dm6    Primary Table: CSE99014_wt_RNH1_untreated_idr    Row Count: 20,404   Data last updated: 2021-09-01
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 16906int(10) unsigned range
chromEnd 17155int(10) unsigned range
name .varchar(255) values
score 757int(10) unsigned range
strand .char(1) values
signalValue 3.2721float range
pValue 12.5899float range
qValue 10.4016float range
peak 133int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L1690617155.757.3.272112.589910.4016133
585chr2L1969219979.1000.3.3705913.532411.2649111
585chr2L110224110434.543.2.714938.516466.7235990
586chr2L168269168871.602.2.537557.074835.43549487
586chr2L168269168871.602.2.820179.363817.48915319
586chr2L171577172085.579.2.136884.605133.26076319
586chr2L171577172085.579.2.820179.363817.48915163
586chr2L174279174696.816.3.5252115.272412.8524126
586chr2L187998188419.709.3.4056613.71211.4238168
586chr2L189607190518.1000.4.9392237.104933.2029541

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

R-Loop DRIP-Seq (S2) (rloopDRIPSeq) Track Description
 

Description

These tracks show the putative locations of R-loops based on DRIP-Seq data from Drosophila melanogaster S2 Cells. The samples that were treated with RNase H served as additional controls for identifying R-loops. The regions that are enriched in the DRIP-Seq reads were identified by MACS2 and Peakzilla. The log likelihood ratio evidence tracks were produced by MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The DRIP-Seq datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession number GSE99014.

References

Bayona-Feliu A, Casas-Lamesa A, Reina O, Bernués J, Azorin F. Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin. Nat Commun. 2017 Aug 18;8(1):283.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.