Schema for R-Loop DRIP-Seq (S2) - R-Loop DRIP-Seq in S2 Cells
  Database: dm6    Primary Table: CSE99014_wt_RNH1_untreated_macs    Row Count: 44,895   Data last updated: 2021-09-01
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 5786int(10) unsigned range
chromEnd 5999int(10) unsigned range
name CSE99014_wt_RNH1_untreated_...varchar(255) values
score 50int(10) unsigned range
strand .char(1) values
signalValue 2.37719float range
pValue 6.60151float range
qValue 5.02341float range
peak 113int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L57865999CSE99014_wt_RNH1_untreated_macs_peak_150.2.377196.601515.02341113
585chr2L80178213CSE99014_wt_RNH1_untreated_macs_peak_235.2.197394.960783.5802252
585chr2L1691517143CSE99014_wt_RNH1_untreated_macs_peak_3104.3.272112.589910.4016124
585chr2L1973819945CSE99014_wt_RNH1_untreated_macs_peak_4112.3.3705913.532411.264965
585chr2L3857138704CSE99014_wt_RNH1_untreated_macs_peak_575.2.871449.436227.5470461
585chr2L3896739228CSE99014_wt_RNH1_untreated_macs_peak_639.2.270445.380953.939154
585chr2L4213642269CSE99014_wt_RNH1_untreated_macs_peak_735.2.156824.897043.5221163
585chr2L5253952673CSE99014_wt_RNH1_untreated_macs_peak_872.2.70359.108917.2563268
585chr2L7367073794CSE99014_wt_RNH1_untreated_macs_peak_923.1.845013.54762.3472345
585chr2L9922799447CSE99014_wt_RNH1_untreated_macs_peak_1023.1.936553.537482.3389232

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

R-Loop DRIP-Seq (S2) (rloopDRIPSeq) Track Description
 

Description

These tracks show the putative locations of R-loops based on DRIP-Seq data from Drosophila melanogaster S2 Cells. The samples that were treated with RNase H served as additional controls for identifying R-loops. The regions that are enriched in the DRIP-Seq reads were identified by MACS2 and Peakzilla. The log likelihood ratio evidence tracks were produced by MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The DRIP-Seq datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession number GSE99014.

References

Bayona-Feliu A, Casas-Lamesa A, Reina O, Bernués J, Azorin F. Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin. Nat Commun. 2017 Aug 18;8(1):283.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.