Schema for R-Loop DRIP-Seq (S2) - R-Loop DRIP-Seq in S2 Cells
  Database: dm6    Primary Table: CSE99014_wt_RNH1_untreated_peakzilla    Row Count: 26,608   Data last updated: 2021-09-01
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 5751int(10) unsigned range
chromEnd 6003int(10) unsigned range
name peakzilla_21967varchar(255) values
score 2int(10) unsigned range
strand .char(1) values
signalValue 3float range
pValue -1float range
qValue 1.51286float range
peak 126int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L57516003peakzilla_219672.3-11.51286126
585chr2L1689417146peakzilla_99023.4.68-11.8729126
585chr2L1967519927peakzilla_128362.3.74-11.80688126
585chr2L5250452756peakzilla_303001.2.28-10.63227126
585chr2L123363123615peakzilla_110853.3.46-11.83565126
586chr2L171613171865peakzilla_229032.2.69-11.46471126
586chr2L174246174498peakzilla_137242.3.55-11.78781126
586chr2L188033188285peakzilla_132162.3.82-11.79317126
586chr2L189157189409peakzilla_234112.2.7-11.43533126
586chr2L190042190294peakzilla_5836.7.46-11.92445126

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

R-Loop DRIP-Seq (S2) (rloopDRIPSeq) Track Description
 

Description

These tracks show the putative locations of R-loops based on DRIP-Seq data from Drosophila melanogaster S2 Cells. The samples that were treated with RNase H served as additional controls for identifying R-loops. The regions that are enriched in the DRIP-Seq reads were identified by MACS2 and Peakzilla. The log likelihood ratio evidence tracks were produced by MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The DRIP-Seq datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession number GSE99014.

References

Bayona-Feliu A, Casas-Lamesa A, Reina O, Bernués J, Azorin F. Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin. Nat Commun. 2017 Aug 18;8(1):283.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.