Schema for RefSeq mRNAs - RefSeq mRNAs mapped to this assembly
  Database: hub_32_GCA_022605925.1    Primary Table: hub_32_xenoRefGene Data last updated: 2023-04-05
Big Bed File: https://hgdownload.soe.ucsc.edu/hubs/GCA/022/605/925/GCA_022605925.1/bbi/GCA_022605925.1_bLatJam1.0.a.xenoRefGene.bb
Item Count: 11,551
Format description: bigGenePred gene models
fieldexampledescription
chromJAKCOY010000001.1Reference sequence chromosome or scaffold
chromStart38279150Start position in chromosome
chromEnd38410722End position in chromosome
nameNM_028803.4RefSeq accession id
score0Score (0-1000)
strand-+ or - for strand
thickStart38279150Start of where display should be thick (start codon)
thickEnd38410722End of where display should be thick (stop codon)
reserved0RGB value (use R,G,B string in input file)
blockCount15Number of blocks
blockSizes45,120,48,183,170,113,4,96,133,123,205,91,133,117,69,Comma separated list of block sizes
chromStarts0,9581,25795,26924,62426,65014,67855,67869,71250,71636,97848,99198,101793,120799,131503,Start positions relative to chromStart
name2Alternative/human readable name
cdsStartStatnoneStatus of CDS start annotation (none, unknown, incomplete, or complete)
cdsEndStatnoneStatus of CDS end annotation (none, unknown, incomplete, or complete)
exonFrames0,0,0,0,1,2,1,1,0,0,2,1,0,0,0,Exon frame {0,1,2}, or -1 if no frame for exon
typeTranscript type
geneNameNM_028803.4gene name
geneName2species of origin of the mRNA
geneTypeGene type

Sample Rows
 
chromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStartsname2cdsStartStatcdsEndStatexonFramestypegeneNamegeneName2geneType
JAKCOY010000001.13827915038410722NM_028803.40-382791503841072201545,120,48,183,170,113,4,96,133,123,205,91,133,117,69,0,9581,25795,26924,62426,65014,67855,67869,71250,71636,97848,99198,101793,120799,131503,nonenone0,0,0,0,1,2,1,1,0,0,2,1,0,0,0,NM_028803.4
JAKCOY010000001.13944325039749474NM_019413.20+394432913974786003796,327,2,156,121,139,128,125,172,206,82,169,167,121,233,122,172,198,2,24,151,314,109,143,54,165,406,153,309,157,16,9,17,21,36,39 ...0,41946,42282,194383,221016,223827,224679,244012,245781,248499,250090,250324,250593,254347,256880,258358,261909,269274,269493,27 ...nonenone0,1,1,0,0,1,2,1,0,1,0,1,2,1,2,1,0,1,1,0,0,1,0,1,0,0,0,1,1,1,2,-1,-1,-1,-1,-1,-1,NM_019413.2
JAKCOY010000001.14003908540197137NM_001378191.10-400414974019713703893,15,109,48,78,222,121,85,155,3,163,66,157,101,109,260,6,111,132,282,128,43,183,172,125,233,121,170,156,4,79,209,172,126,127,13 ...0,966,1308,1494,1802,1927,2189,2329,3503,8088,8114,8310,15619,30014,30124,33446,35025,40792,45376,45878,50227,51902,61197,67002, ...nonenone-1,-1,-1,-1,-1,-1,-1,1,2,2,1,1,0,1,0,1,1,1,1,1,2,1,1,0,1,2,1,2,2,1,0,1,0,0,2,1,0,0,NM_001378191.1
JAKCOY010000001.14069350440699270NM_174298.40+406935044069927006117,138,51,26,158,90,0,854,4991,5099,5498,5676,nonenone0,0,0,0,2,1,NM_174298.4
JAKCOY010000001.14074830040765933NM_204737.10-407490184076592701184,169,72,36,102,36,170,59,4,534,130,0,117,298,426,550,662,709,1322,3824,4649,17503,nonenone-1,-1,-1,-1,-1,-1,1,2,1,1,0,NM_204737.1
JAKCOY010000001.14093726440948183NM_001126877.10+409372644094818305309,167,45,104,85,0,1076,4535,6635,10834,nonenone0,0,2,2,1,NM_001126877.1
JAKCOY010000001.14095374941006961NM_174439.20-4095374941006961015138,141,84,174,134,76,136,119,95,154,14,71,145,123,87,0,1812,2888,5270,15264,18074,20969,21561,22924,28674,29311,29343,31776,42643,53125,nonenone2,2,2,2,0,2,1,2,0,2,0,1,0,0,0,NM_174439.2
JAKCOY010000001.14219355042483423NM_203409.10+421935814248342302176,75,3,210,138,137,29,157,146,150,188,87,175,5,117,120,177,9,200,121,111,0,157229,157322,158057,160512,165943,173179,178170,186740,201224,214674,216171,225394,235997,245792,248651,255367,255672,279235, ...nonenone0,0,0,0,0,0,2,1,2,1,1,0,0,1,0,0,0,0,0,2,0,NM_203409.1
JAKCOY010000001.14408020644088680NM_001011362.10-44080206440886800751,153,105,65,100,138,141,0,931,4493,5927,7273,7861,8333,nonenone0,0,0,1,0,0,0,NM_001011362.1
JAKCOY010000001.14410383644142193NM_001006257.10+44103859441420450876,66,113,206,102,108,32,181,0,23451,28377,31653,33333,35128,37054,38176,nonenone0,2,2,1,0,0,0,2,NM_001006257.1

RefSeq mRNAs (hub_32_xenoRefGene) Track Description
 

Description

The RefSeq mRNAs gene track for the 17 Mar 2022 Laterallus jamaicensis coturniculus/GCA_022605925.1_bLatJam1.0.a genome assembly displays translated blat alignments of vertebrate and invertebrate mRNA in GenBank.

Data Access

Download GCA_022605925.1_bLatJam1.0.a.xenoRefGene.gtf.gz GTF file.

Track statistics summary

Total genome size: 1,211,372,722
Gene count: 11,551
Bases in genes: 379,537,567
Percent genome coverage: % 31.331

Methods

The mRNAs were aligned against the Laterallus jamaicensis coturniculus/GCA_022605925.1_bLatJam1.0.a genome using translated blat. When a single mRNA aligned in multiple places, the alignment having the highest base identity was found. Only those alignments having a base identity level within 1% of the best and at least 25% base identity with the genomic sequence were kept.

Specifically, the translated blat command is:

blat -noHead -q=rnax -t=dnax -mask=lower target.fa query.fa target.query.psl

where target.fa is one of the chromosome sequence of the genome assembly,
and the query.fa is the mRNAs from RefSeq
The resulting PSL outputs are filtered:
pslCDnaFilter -minId=0.35 -minCover=0.25  -globalNearBest=0.0100 -minQSize=20 
  -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap 
    all.results.psl GCA_022605925.1_bLatJam1.0.a.xenoRefGene.psl
The filtered GCA_022605925.1_bLatJam1.0.a.xenoRefGene.psl is converted to genePred data to display for this track.

Credits

The mRNA track was produced at UCSC from mRNA sequence data submitted to the international public sequence databases by scientists worldwide.

References

Benson DA, Cavanaugh M, Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW. GenBank. Nucleic Acids Res. 2013 Jan;41(Database issue):D36-42. PMID: 23193287; PMC: PMC3531190

Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL. GenBank: update. Nucleic Acids Res. 2004 Jan 1;32(Database issue):D23-6. PMID: 14681350; PMC: PMC308779

Kent WJ. BLAT - the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64. PMID: 11932250; PMC: PMC187518