Schema for NCBI RefSeq - RefSeq gene predictions from NCBI
  Database: hub_32_GCF_000710305.1    Primary Table: hub_32_ncbiRefSeqPsl Data last updated: 2020-11-29
Big Bed File: https://hgdownload.soe.ucsc.edu/hubs/GCF/000/710/305/GCF_000710305.1/bbi/GCF_000710305.1_ASM71030v1.bigPsl.bb
Item Count: 15,595
Format description: bigPsl pairwise alignment
fieldexampledescription
chromNW_010387642.1Reference sequence chromosome or scaffold
chromStart2392Start position in chromosome
chromEnd26095End position in chromosome
nameXM_010144529.1Name or ID of item, ideally both human readable and unique
score1000Score (0-1000)
strand-+ or - indicates whether the query aligns to the + or - strand on the reference
thickStart2392Start of where display should be thick (start codon)
thickEnd26095End of where display should be thick (stop codon)
reserved0RGB value (use R,G,B string in input file)
blockCount10Number of blocks
blockSizes138,165,108,98,99,85,167,150,178,126,Comma separated list of block sizes
chromStarts0,7975,12429,13253,16701,20092,21063,21974,23000,23577,Start positions relative to chromStart
oChromStart0Start position in other chromosome
oChromEnd1314End position in other chromosome
oStrand++ or -, - means that psl was reversed into BED-compatible coordinates
oChromSize1314Size of other chromosome.
oChromStarts0,138,303,411,509,608,693,860,1010,1188,Start positions relative to oChromStart or from oChromStart+oChromSize depending on strand
oSequenceatggatggtacaaaggggagcttggataaggttttaaggcctacatctagctcaagagcttcccctggctgtagagctttaaatacctctatggagatactgacagcagagccacgttcaccagaggttggttcacctatcagttgggctggtagtcatcaaagagtcagtctaacagactgctgtccggaagaaaacaaaggtggtgacagagagaactctgctgccaaggaagttagaacagagcagcaaaccatgaccacctttcttcagaagcgtgcgttggaccccagggacttgccaggatgtatctctaattcagattctggagatgacagtgctgaaacacagacttcaaaattctgtgaatcttcccgaggaaaactaagcgatgcaagagcaggtattacttttcaggtgtataaagtgaacagccagtgcaatgaaacttcttcttccaacttggaatattctgaagaagcagttgtagagcctcagattcaagcagctatgaagaaaatgaataaacttgatacagtattggcaaaaaaactctttaaggagagagcaattaaaaaacaaggcaaagaaatgagggcgaagctctgggaagaacttcagtctatcggaaccactggaagccaggaggaggtagaaaacacaaaactttttttagctttgatctcatcatggcaggatacagttgatccctccggtgctgaagatgctgaaatacatacttcagtatttcacacagaaattaatccagaacgttacgattgccataaaccccaacctaatcaggatcacccaagtgaattagaaacaaacgaatttagtcaagcagaaaaaacgcacagcaaaagtaaaaacaatcaggatttcattaaaaagaacatcgagctagcaaaggattcaggaagccagattgttatgcttgagagagaaaggaaaagactaattgaactattgaaagatacagaagatggaactgaattgcaaggtcttgaggaggatgaatcttgctggctagcaccaggagaagggtacacacctgaaccaatggaatttcaccacctaaatgagatagacattaagcttcaagtactacagtctgatggggatttttctgctatttccagctcttgctcaaaatctccaggccagctttatcaggagtctctggcttatgcgaaccgaagcctagaagcagttccaggtgaaaaggttctgcgagacaataaggagcaacgtgatcagcaaaatcgtctgaaagaaattgatcatcagctgaagtccctagagagaaatcgtSequence on other chrom (or edit list, or empty)
oCDS1..>1314CDS in NCBI format
chromSize26730Size of target chromosome
match1086Number of bases matched.
misMatch0 Number of bases that don't match
repMatch228 Number of bases that match but are part of repeats
nCount0 Number of 'N' bases
seqType10=empty, 1=nucleotide, 2=amino_acid

Sample Rows
 
chromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStartsoChromStartoChromEndoStrandoChromSizeoChromStartsoSequenceoCDSchromSizematchmisMatchrepMatchnCountseqType
NW_010387642.1239226095XM_010144529.11000-239226095010138,165,108,98,99,85,167,150,178,126,0,7975,12429,13253,16701,20092,21063,21974,23000,23577,01314+13140,138,303,411,509,608,693,860,1010,1188,atggatggtacaaaggggagcttggataaggttttaaggcctacatctagctcaagagcttcccctggctgtagagctttaaatacctctatggagatactgacagcagagccacgttcaccagaggt ...1..>1314267301086022801

RefSeq Alignments (hub_32_ncbiRefSeqPsl) Track Description
 

Description

The NCBI RefSeq Genes composite track shows 25 Jun 2014 Buceros rhinoceros silvestris/GCF_000710305.1_ASM71030v1 protein-coding and non-protein-coding genes taken from the NCBI RNA reference sequences collection (RefSeq). All subtracks use coordinates provided by RefSeq. See the Methods section for more details about how the different tracks were created.

Please visit NCBI's Feedback for Gene and Reference Sequences (RefSeq) page to make suggestions, submit additions and corrections, or ask for help concerning RefSeq records.

For more information on the different gene tracks, see our Genes FAQ.

Data Access

Download GCF_000710305.1_ASM71030v1.ncbiRefSeq.gtf.gz GTF file.

Display Conventions and Configuration

To show only a selected set of subtracks, uncheck the boxes next to the tracks that you wish to hide.

The tracks available here can include (not all may be present):
RefSeq annotations and alignments
  • RefSeq All – all curated and predicted annotations provided by RefSeq.
  • RefSeq Curated – subset of RefSeq All that includes only those annotations whose accessions begin with NM, NR, NP or YP. (NP and YP are used only for protein-coding genes on the mitochondrion; YP is used for human only.)
  • RefSeq Predicted – subset of RefSeq All that includes those annotations whose accessions begin with XM or XR.
  • RefSeq Other – all other annotations produced by the RefSeq group that do not fit the requirements for inclusion in the RefSeq Curated or the RefSeq Predicted tracks.
  • RefSeq Alignments – alignments of RefSeq RNAs to the 25 Jun 2014 Buceros rhinoceros silvestris/GCF_000710305.1_ASM71030v1 genome provided by the RefSeq group.

The RefSeq All, RefSeq Curated and RefSeq Predicted, tracks follow the display conventions for gene prediction tracks. The color shading indicates the level of review the RefSeq record has undergone: predicted (light), provisional (medium), or reviewed (dark), as defined by RefSeq.

Color Level of review
Reviewed: the RefSeq record has been reviewed by NCBI staff or by a collaborator. The NCBI review process includes assessing available sequence data and the literature. Some RefSeq records may incorporate expanded sequence and annotation information.
Provisional: the RefSeq record has not yet been subject to individual review. The initial sequence-to-gene association has been established by outside collaborators or NCBI staff.
Predicted: the RefSeq record has not yet been subject to individual review, and some aspect of the RefSeq record is predicted.

The RefSeq Alignments track follows the display conventions for PSL tracks.

The item labels and codon display properties for features within this track can be configured through the controls at the top of the track description page. To adjust the settings for an individual subtrack, click the wrench icon next to the track name in the subtrack list.

  • Label: By default, items are labeled by gene name. Click the appropriate Label option to display the accession name or OMIM identifier instead of the gene name, show all or a subset of these labels including the gene name, OMIM identifier and accession names, or turn off the label completely.
  • Codon coloring: This track has an optional codon coloring feature that allows users to quickly validate and compare gene predictions. To display codon colors, select the genomic codons option from the Color track by codons pull-down menu. For more information about this feature, go to the Coloring Gene Predictions and Annotations by Codon page.

Methods

The RefSeq annotation and RefSeq RNA alignment tracks were created at UCSC using data from the NCBI RefSeq project. GFF format data files were downloaded from the file GCF_000710305.1_ASM71030v1_genomic.gff.gz delivered with the NCBI RefSeq genome assemblies at the FTP location:
ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/710/305/GCF_000710305.1_ASM71030v1/ The GFF file was converted to the genePred and PSL table formats for display in the Genome Browser. Information about the NCBI annotation pipeline can be found here.

Track statistics summary

Total genome size: 1,065,782,791 bases

Curated and Predicted Gene count: 15,595
Bases in these genes: 253,449,600
Percent genome coverage: % 23.781

There are no curated gene annotations.

Predicted gene count: 15,595
Bases in genes: 253,449,600
Percent genome coverage: % 23.781

Other annotation count: 207
Bases in other annotations: 480,113
Percent genome coverage: % 0.045

Credits

This track was produced at UCSC from data generated by scientists worldwide and curated by the NCBI RefSeq project.

References

Kent WJ. BLAT - the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64. PMID: 11932250; PMC: PMC187518

Pruitt KD, Brown GR, Hiatt SM, Thibaud-Nissen F, Astashyn A, Ermolaeva O, Farrell CM, Hart J, Landrum MJ, McGarvey KM et al. RefSeq: an update on mammalian reference sequences. Nucleic Acids Res. 2014 Jan;42(Database issue):D756-63. PMID: 24259432; PMC: PMC3965018

Pruitt KD, Tatusova T, Maglott DR. NCBI Reference Sequence (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins. Nucleic Acids Res. 2005 Jan 1;33(Database issue):D501-4. PMID: 15608248; PMC: PMC539979