Inhibit Topoisomerases PolII ChIP-Seq Track Settings

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PolII ChIP-Seq After Inhibition of Topoisomerases (All ChIP Seq Tracks)

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	hide Configure	Control PolII MACS	PolII MACS Peaks (Control)	Schema
	hide Configure	PolII LogLR (Control)	PolII Log Likelihood Enrichment (Control)	Schema
	hide Configure	Inhibit Top1 PolII IDR	PolII IDR Peaks After Inhibition of Top1	Schema
	hide Configure	Inhibit Top1 PolII MACS	PolII MACS Peaks After Inhibition of Top1	Schema
	hide Configure	Inhibit Top1 PolII logLR	PolII Log Likelihood Enrichment After Inhibition of Top1	Schema
	hide Configure	Inhibit Top2 PolII IDR	PolII IDR Peaks After Inhibition of Top2	Schema
	hide Configure	Inhibit Top2 PolII MACS	PolII MACS Peaks After Inhibition of Top2	Schema
	hide Configure	Inhibit Top2 PolII logLR	PolII Log Likelihood Enrichment After Inhibition of Top2	Schema

Description

These tracks show the regions in the D. melanogaster genome that are significantly enriched in RNA Polymerase II (PolII) in S2 cells as determined by MACS2. The samples were treated with Camptothecin to inhibit topoisomerase I (Top1), and treated with ICRF-193 to inhibit topoisomerase II (Top2). The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The PolII ChIP-Seq datasets were obtained from the NCBI Gene Expression Omnibus database under the accession number GSE47795.

References

Teves SS, Henikoff S. Transcription-generated torsional stress destabilizes nucleosomes. Nat Struct Mol Biol. 2014 Jan;21(1):88-94.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137. doi: 10.1186/gb-2008-9-9-r137. Epub 2008 Sep 17.