MNase Accessibility (R5) Track Settings

MNase Accessibility of S2 Cells (R5) (All MNase Tracks)

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	hide Configure	h-MACC (H3)	Histone 3 h-MACC in S2 Cells	Schema
	hide Configure	h-MACC (H4)	Histone 4 h-MACC in S2 Cells	Schema
	hide Configure	c-MACC (Chromatin)	Whole Chromatin c-MACC in S2 Cells	Schema
	hide Configure	Pooled (H3)	Pooled Histone 3 MNase Accessibility in S2 Cells	Schema
	hide Configure	Pooled (H4)	Pooled Histone 4 MNase Accessibility in S2 Cells	Schema
	hide Configure	Pooled (Chromatin)	Pooled Whole Chromatin MNase Accessibility in S2 Cells	Schema

Description

These tracks show the micrococcal nuclease (MNase) accessibility (MACC) of the D. melanogaster genome. Chromatin from D. melanogaster were digested using four different concentrations of MNase (1.5, 6.25, 25, and 100U). The digested DNA fragments are either sequenced as whole-chromatin extract (c-MACC), or after chromatin immunoprecipitation of histones H3 and H4 (h-MACC). The pooled evidence tracks were constructed by averaging the signals from the four concentrations of MNase.

Credits

The datasets were retrieved from the Gene Expression Omnibus database at NCBI under the accession number GSE78984. The results were lifted from the D. melanogaster release 5 assembly to the release 6 assembly.

Mieczkowski J, Cook A, Bowman SK, Mueller B, Alver BH, Kundu S, Deaton AM, Urban JA, Larschan E, Park PJ, Kingston RE, Tolstorukov MY. MNase titration reveals differences between nucleosome occupancy and chromatin accessibility. Nat Commun. 2016 May 6;7:11485.