Core LJ 2012 GRO-Seq (R5) Track Settings

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GRO-Seq for Drosophila S2 Cells (Core LJ, 2012) (R5) (All Expression and Regulation tracks)

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	hide Configure	GRO-Seq Untreated (Plus)	GRO-Seq Untreated Merged Sample GSM577244 (Plus)	Schema
	hide Configure	GRO-Seq Untreated (Minus)	GRO-Seq Untreated Merged Sample GSM577244 (Minus)	Schema
	hide Configure	GRO-Seq Mock RNAi (Plus)	GRO-Seq Mock RNAi Merged Sample GSM577245 (Plus)	Schema
	hide Configure	GRO-Seq Mock RNAi (Minus)	GRO-Seq Mock RNAi Merged Sample GSM577245 (Minus)	Schema
	hide Configure	GRO-Seq NELF RNAi (Plus)	GRO-Seq NELF RNAi Merged Sample GSM577246 (Plus)	Schema
	hide Configure	GRO-Seq NELF RNAi (Minus)	GRO-Seq NELF RNAi Merged Sample GSM577246 (Minus)	Schema
	hide Configure	GRO-Seq without Sarkosyl (Plus)	GRO-Seq without Sarkosyl Sample GSM577248 (Plus)	Schema
	hide Configure	GRO-Seq without Sarkosyl (Minus)	GRO-Seq without Sarkosyl Sample GSM577248 (Minus)	Schema
	hide Configure	GRO-Seq with Sarkosyl (Plus)	GRO-Seq with Sarkosyl Sample GSM577247 (Plus)	Schema
	hide Configure	GRO-Seq with Sarkosyl (Minus)	GRO-Seq with Sarkosyl Sample GSM577247 (Minus)	Schema

Description

These tracks show the read coverage from global run-on sequencing (GRO-Seq) of D. melanogaster S2 cells. The NELF RNAi sample was treated with double-stranded RNAs (dsRNAs) targeting NELF-B and NELF-E. The mock RNAi sample was treated with dsRNAs against β-galactosidase. Treatment with the anionic detergent Sarkosyl results in the resumption of transcription by paused polymerases.

Credits

The datasets were retrieved from the Gene Expression Omnibus database at NCBI under the accession number GSE23544. The results were lifted from the D. melanogaster release 5 assembly to the release 6 assembly.

Core LJ, Waterfall JJ, Gilchrist DA, Fargo DC, Kwak H, Adelman K, Lis JT. Defining the status of RNA polymerase at promoters. Cell Rep. 2012 Oct 25;2(4):1025-35.