Description
This track shows the aggregated RNA-Seq coverage for D. hydei. The RNA-Seq reads were mapped against the D. hydei (DhydRefSeq1) genome assembly using HISAT2. RNA-Seq read alignments from multiple replicates were combined together, and the read coverage was normalized by a wigsum of 100,000,000 (1 million 100bp reads). The read coverage tracks were produced by the bam2wig.py program in RSeQC. These RNA-Seq read coverage tracks were then combined using the bigWigMerge tool developed by the UCSC Genome Browser Project Team to create the aggregated RNA-Seq coverage track.
Accession Numbers for RNA-Seq Data
The RNA-Seq data were obtained from NCBI using the following accession numbers:
References
Zhao L, Begun DJ. Genomics
of parallel adaptation at two timescales in Drosophila. PLoS Genet. 2017
Oct 2;13(10):e1007016.
Kim D, Langmead B, Salzberg SL. HISAT: a
fast spliced aligner with low memory requirements. Nat Methods. 2015 Apr;12(4):357-60.
Wang L, Wang S, Li W. RSeQC: quality
control of RNA-Seq experiments. Bioinformatics. 2012 Aug 15;28(16):2184-5.
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